Related Experiment Video
Updated: Jul 6, 2026
Improved Genome Editing via Oviductal Nucleic Acids Delivery-based In Vivo Electroporation Technique for Knockout Mice Generation
Published on: August 26, 2025
[Construction of CyclinD (-/-)/Patched (+/-) double knockout mice]
Shi-Jie Xin1, Xuan Li, Duman-Scheel Molly
1Department of Vascular Surgery, First Hospital of China Medical University, Shenyang 110001, China. xinshijie1963@yahoo.com.cn
Objective:
To construct CyclinD (CycD) (-/-)/Patched (Ptc) (+/-) double knockout mice to study the relationship between Hedgehog and Rb signaling pathways.
Methods:
Amphimixis was performed between female heterozygote CycD knockout mice CycD (+/-) and male heterozygote Ptc knockout mice Ptc (+/-), thus CycD (+/-)/Ptc (+/-) double knockout mice were obtained. These mice underwent secondary matched and thus construct CycD (-/-)/Ptc (+/-) double knockout mice.
Results:
CycD (-/-)/Ptc (+/-) double knockout mice, CycD (+/-)/Ptc (+/-) heterozygote double knockout mice, and CycD knockout mice CycD (-/-)/Ptc (+/+) were gained.
Conclusion:
The method of amphimixis can construct CycD (--)/Ptc (+/-) double knockout mice that can be used to disclose the regulation between Ptc gene and CycD gene and the relativity between Hedgehog and Rb pathways in mammals.
Insights
Researchers created CyclinD (CycD) knockout and Patched (Ptc) heterozygous mice to study Hedgehog and Rb pathway interactions. This model enables investigation into the Ptc gene
Area of Science:
- Developmental Biology
- Genetics
- Molecular Biology
Background:
- The Hedgehog signaling pathway, regulated by Patched (Ptc), plays a crucial role in mammalian development.
- CyclinD (CycD) is a key regulator of the cell cycle and is implicated in the Retinoblastoma (Rb) pathway.
- Dysregulation of these pathways is associated with various developmental abnormalities and cancers.
Purpose of the Study:
- To generate a novel mouse model, CyclinD (CycD) knockout/Patched (Ptc) heterozygous (CycD-/-/Ptc+/-), for studying the interplay between Hedgehog and Rb signaling.
- To investigate the regulatory relationship between the Ptc gene and the CycD gene within this model.
Main Methods:
- Amphimixis was employed to cross female CycD heterozygous (CycD+/-) mice with male Ptc heterozygous (Ptc+/-) mice.
- Subsequent breeding of the resulting double heterozygous offspring (CycD+/-/Ptc+/-) was performed to obtain the desired CycD-/-/Ptc+/- double knockout mice.
Main Results:
- The breeding strategy successfully produced CycD-/-/Ptc+/- double knockout mice.
- Heterozygous double knockout mice (CycD+/-/Ptc+/-) and CycD knockout mice (CycD-/-/Ptc+/+) were also obtained.
Conclusions:
- Amphimixis is an effective method for constructing CycD-/-/Ptc+/- double knockout mice.
- This mouse model provides a valuable tool for elucidating the regulatory interactions between Ptc and CycD.
- The model will facilitate further research into the relationship between Hedgehog and Rb signaling pathways in mammals.
