Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sequence of a cDNA encoding a mouse cyclin B protein.

G D Paterno1, K M Downs

  • 1Terry Fox Cancer Research Laboratory, Faculty of Medicine, Memorial University, St. John's, Newfoundland, Canada.

Gene
|December 15, 1991
PubMed
Summary

Researchers sequenced a mouse cyclin B gene, revealing its genetic makeup. The resulting protein shares significant similarity with human and frog cyclin B variants.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Analysis of the effect of radiation repair mutations on the DEL1 mutator region of Saccharomyces cerevisiae.

Current genetics·2013
Same author

Changes in subcellular localisation of MI-ER1 alpha, a novel oestrogen receptor-alpha interacting protein, is associated with breast cancer progression.

British journal of cancer·2008
Same author

Early placental ontogeny in the mouse.

Placenta·2002
Same author

Nuclear localization signals in the Xenopus FGF embryonic early response 1 protein.

FEBS letters·2001
Same author

Study of the murine allantois by allantoic explants.

Developmental biology·2001
Same author

Developmentally dynamic histone acetylation pattern of a tissue-specific chromatin domain.

Proceedings of the National Academy of Sciences of the United States of America·2000

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Cyclins are critical regulators of the cell cycle.
  • Cyclin B proteins are key components of maturation promoting factor (MPF).
  • Understanding cyclin B diversity across species aids in elucidating cell cycle control mechanisms.

Purpose of the Study:

  • To determine the nucleotide sequence of a mouse cyclin B protein.
  • To analyze the evolutionary conservation of mouse cyclin B.

Main Methods:

  • cDNA sequencing
  • Bioinformatic analysis for protein similarity comparisons.

Main Results:

  • The nucleotide sequence of mouse (Mus musculus) cyclin B was determined.
  • The deduced mouse cyclin B protein exhibited 84% similarity to human cyclin.
  • Significant similarity was also observed with Xenopus cyclin B1 (66%) and B2 (49%).

Conclusions:

  • The mouse cyclin B sequence provides a valuable resource for cell cycle research.
  • The high degree of similarity suggests conserved functions in cell cycle regulation between mammals and amphibians.
  • Further studies can explore functional differences and similarities in cyclin B across species.

Related Experiment Videos