Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

B lymphocyte fibronectin receptors: expression and utilization.

D G Stupack1, S Stewart, W G Carter

  • 1Rheumatic Diseases Unit Research Laboratory, University of Manitoba, Winnipeg, Canada.

Scandinavian Journal of Immunology
|December 1, 1991
PubMed
Summary

Human B cells utilize specific fibronectin receptors (FNR) for adhesion. Activated B cells, like T cells, express alpha 5 beta 1 integrin for fibronectin binding.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

TIFA, an inflammatory signaling adaptor, is tumor suppressive for liver cancer.

Oncogenesis·2015
Same author

Triggering necroptosis in cisplatin and IAP antagonist-resistant ovarian carcinoma.

Cell death & disease·2014
Same author

Knockdown of specific host factors protects against influenza virus-induced cell death.

Cell death & disease·2013
Same author

EGFR-dependent pancreatic carcinoma cell metastasis through Rap1 activation.

Oncogene·2011
Same author

Dynamics of plasma proteome during leptin-replacement therapy in genetically based leptin deficiency.

The pharmacogenomics journal·2010
Same author

Paclitaxel promotes a caspase 8-mediated apoptosis through death effector domain association with microtubules.

Oncogene·2009

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Lymphocyte adhesion to fibronectin (FN) is mediated by very late antigen (VLA) family receptors.
  • The precise roles of diverse fibronectin receptors (FNR) in lymphocyte function remain incompletely understood.
  • Potential functions include differential signaling, enhanced cell attachment, and regulation of cell homing and retention.

Purpose of the Study:

  • To investigate the fibronectin adherence properties of human B cells.
  • To determine the specific fibronectin receptors (FNR) utilized by human B cells.
  • To compare FNR expression and utilization between resting and activated B cells.

Main Methods:

  • Analysis of fibronectin adherence using ten human B-cell lines.
  • Characterization of FNR expression and utilization patterns in B-cell lines and primary cells.

Related Experiment Videos

  • Assessment of B-cell adherence to fibronectin-coated surfaces following in vitro stimulation.

    • Main Results:

    • Six of ten B-cell lines exhibited significant fibronectin adherence.
    • Adherent B-cell lines primarily used alpha 4 beta 1 or a combination of alpha 4 beta 1 and alpha 5 beta 1 integrins.
    • Freshly isolated B cells showed minimal fibronectin adherence, which increased upon activation and was associated with enhanced VLA antigen expression.

    Conclusions:

    • Activated human B cells, analogous to T cells, can express and utilize alpha 5 beta 1 as a fibronectin receptor.
    • B-cell activation leads to the acquisition of fibronectin-binding capacity.
    • The study highlights the dynamic regulation of fibronectin receptor expression and function in B lymphocytes.