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[Analysis of clinical features and virological characteristics in patients infected with three different HBV
Bin Zhou1, Zhan-hui Wang, Jin-jun Chen
1Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, China.
Insights
Hepatitis B virus (HBV) subgenotypes Ba, C1, and C2 exhibit distinct precore and core promoter mutation patterns. These variations influence hepatitis B e antigen-negative infections across different HBV subgenotypes.
Area of Science:
- Hepatology and Virology: Focus on viral genetics and clinical manifestations.
- Molecular Biology: Analysis of specific gene mutations in Hepatitis B Virus.
Context:
- Chronic Hepatitis B Virus (HBV) infection is a global health concern.
- Understanding HBV subgenotypes and their associated mutations is crucial for disease management.
- Previous research has identified various HBV mutations, but their differential impact across subgenotypes requires further investigation.
Purpose:
- To investigate the clinical characteristics and mutation patterns in the precore and core promoter regions of HBV subgenotypes Ba, C1, and C2.
- To correlate specific HBV mutations with clinical outcomes and subgenotype prevalence in a Chinese cohort.
Summary:
- A study of 151 chronic HBV patients in China analyzed HBV subgenotypes (Ba, C1, C2) and mutations.
- No significant clinical differences were observed among subgenotypes regarding HBeAg positivity or liver function.
- HBV/Ba showed high A1896 mutation but low T1762/A1764 mutation; HBV/C1 showed the opposite; HBV/C2 was intermediate.
Impact:
- Identifies distinct mutation profiles for HBV subgenotypes Ba, C1, and C2.
- Provides insights into the molecular mechanisms underlying HBeAg-negative HBV infections.
- Contributes to a better understanding of HBV pathogenesis and potential therapeutic targets.
Objective:
To investigate the clinical characteristics and the pattern of precore and core promoter mutations of hepatitis B virus (HBV) subgenotypes Ba, C1 and C2.
Methods:
A cohort of 151 patients with chronic HBV infection in Guangdong province of China was enrolled in this study. HBV subgenotypes were determined by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). Precore and core promoter mutations were analysed using nucleotide sequencing.
Results:
Of the 151 patients, 80, 51 and 20 were infected with subgenotypes Ba, C1 and C2 respectively. No significant differences were found in HBeAg positivity and liver functional indexes among these three subgenotypes when age and sex were matched. Virologically, HBV/Ba showed the highest frequency of A1896 mutation but the lowest frequency of T1762/A1764 mutation. HBV/C1 was associated with the highest tendency to develop T1762/A1764 mutation, but the lowest prevalence of A1896 mutation. HBV/C2 was associated with an intermediate tendency to develop A1896 and T1762/A1764 mutations.
Conclusion:
Different mutation patterns in precore and core promoter regions are responsible for HBeAg-negative HBV infections among different subgenotypes.
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