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Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

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Radiative transport-based frequency-domain fluorescence tomography.

Amit Joshi¹, John C Rasmussen, Eva M Sevick-Muraca

¹Division of Molecular Imaging, Department of Radiology, Baylor College of Medicine, Houston, TX 77030, USA. amitj@bcm.edu

Physics in Medicine and Biology

|March 28, 2008

Summary

We developed a new fluorescence optical tomography method using radiative transport models. This technique efficiently locates fluorophore concentrations in simulated mouse organs with minimal computational cost.

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Area of Science:

Biomedical optics
Medical imaging
Computational modeling

Background:

Fluorescence optical tomography (FOT) is a promising imaging modality for biological tissues.
Accurate modeling of near-infrared (NIR) fluorescence propagation is crucial for FOT.
Existing methods often face challenges with computational cost and accuracy.

Purpose of the Study:

To develop a novel radiative transport model-based FOT method.
To enable efficient and accurate prediction of fluorescence measurements.
To demonstrate the capability of locating fluorophore concentrations in biological tissues.

Main Methods:

Utilized a coupled radiative transport model for NIR fluorescence propagation.
Employed a novel software based on the Attila particle transport simulation platform.
Implemented an adjoint transport solution-based algorithm on dual grids for Jacobian matrix assembly.

Main Results:

Achieved prediction of fluorescence measurements with non-contact sources and detectors.
Demonstrated minimal computational cost for the proposed scheme.
Successfully performed fluorescence tomography on a realistic computational mouse model.

Conclusions:

The developed radiative transport model-based FOT is effective for locating fluorophore distributions.
The method offers a computationally efficient approach for biomedical imaging.
This technique shows potential for in vivo imaging applications in preclinical research.