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Updated: Jul 6, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
Published on: May 30, 2016
Combination of grating lenses for color splitting and imaging
Abstract:
A pair of planar reflection gratings is proposed and discussed for use in a color-splitting and imaging system, such as a projection-type color-scanner head. Red, green, and blue light reflected from a color subject are split by a color-splitting grating lens and imaged by an image-correction grating, consisting of three segments, onto three line sensors arranged in parallel. The phase-shift function of the image-correction grating was optimized for each color by numerical iteration with the ray-tracing method to suppress aberrations resulting from the wide view angle and the three different wavelengths. Gratings were designed and fabricated, and aberration suppression was experimentally confirmed.
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