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Imaging Biological Samples with Optical Microscopy

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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Related Experiment Video

Updated: Jul 6, 2026

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

Interline Transfer CCD Camera for Gated Broadband Coherent Anti-Stokes Raman-Scattering Measurements.

S Roy, G Ray, R P Lucht

|March 28, 2008

Summary

This summary is machine-generated.

This study demonstrates using an interline transfer CCD camera for Coherent Anti-Stokes Raman Scattering (CARS) spectroscopy. This new method enables rapid, gated single-laser-shot CARS measurements, offering improved performance compared to traditional CCD cameras.

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Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering (CARS)

Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering (CARS)

Published on: October 17, 2010

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Last Updated: Jul 6, 2026

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering (CARS)

Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering (CARS)

Published on: October 17, 2010

Area of Science:

Spectroscopy
Optical Physics
Chemical Analysis

Background:

Coherent Anti-Stokes Raman Scattering (CARS) is a powerful spectroscopic technique.
Acquiring CARS spectra typically requires specialized equipment for high-speed measurements.
Interline transfer CCD cameras offer unique capabilities for rapid image acquisition.

Purpose of the Study:

To demonstrate the utility of an interline transfer CCD camera for broadband CARS spectroscopy.
To evaluate the performance of this camera system for gated CARS measurements.
To compare the interline transfer CCD camera with a standard back-illuminated CCD camera for CARS applications.

Main Methods:

Utilizing the dual-image mode of an interline transfer CCD camera.
Acquiring CARS spectral images and rapidly shifting them to storage pixels for gating.
Performing gated single-laser-shot CARS measurements on hydrogen and nitrogen.
Comparing camera performance in room temperature and flame environments.

Main Results:

Successful acquisition of broadband CARS spectra using the interline transfer CCD camera.
Demonstrated effective gated CARS measurements with single-laser-shot capability.
Observed comparable or improved performance of the interline transfer CCD camera relative to a standard CCD.

Conclusions:

Interline transfer CCD cameras are suitable for high-performance, gated CARS spectroscopy.
The dual-image mode enables rapid spectral acquisition and gating for transient species.
This approach offers a viable alternative for CARS measurements, especially in challenging environments.