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Updated: Jul 6, 2026

In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines
Published on: May 12, 2023
1H NMR study of the enantioselective binding of lambda- and delta-[Ru(bpy)2(m-bpy-GHK)]Cl2 to the deoxynucleotide
A Myari1, N Hadjiliadis, A Garoufis
1Laboratory of Inorganic and General Chemistry, Department of Chemistry, University of Ioannnina, Ioannina 45110, Greece.
Abstract:
The interaction of the diastereomeric complexes Lambda- and Delta - [Ru(bpy)(2)(m - GHK)]Cl(2), (GHK = glycine-histidine-lysine) to the deoxynucleotide duplex d(5'-CGCGAATTCGCG-3')(2) was studied by means of (1)H NMR spectroscopy. The diastereomers interact with the oligonucleotide duplex differently. The Delta - [Ru(bpy)(2) (m - GHK)]Cl(2) is characterized by major groove binding close to the central part of the oligonucleotide, with both the peptide and the bipyridine ligand of the complex involved in the binding. The lambda - [Ru(bpy)(2) (m - bpy - GHK)]C(2) binds loosely, approaching the helix from the minor groove. The NMR analysis shows that the peptide (GHK) binding has a determinative role in the interactions of both diastereomers with the oligonucleotide.
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