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Related Experiment Video

Updated: Jul 6, 2026

Light Spot-Based Assay for Analysis of Drosophila Larval Phototaxis

Light Spot-Based Assay for Analysis of Drosophila Larval Phototaxis

Published on: September 27, 2019

UV-absorbance detector for HPLC based on a light-emitting diode.

Stefan Schmid¹, Mirek Macka, Peter C Hauser

¹Department of Chemistry, University of Basel, Spitalstrasse 51, 4056, Basel, Switzerland.

|March 28, 2008

Summary

A new deep-UV optical absorption detector for High-Performance Liquid Chromatography (HPLC) was developed using a novel light-emitting diode. This cost-effective detector demonstrates excellent performance for analyzing various chemical compounds.

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Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared

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Published on: January 10, 2025

Area of Science:

Analytical Chemistry
Spectroscopy
Chromatography

Background:

Traditional optical absorption detectors for High-Performance Liquid Chromatography (HPLC) can be complex and expensive.
There is a need for cost-effective and sensitive detection methods in HPLC analysis.
Deep-ultraviolet (UV) detection is crucial for analyzing many organic compounds.

Purpose of the Study:

To construct and evaluate a novel flow-through optical absorption detector for HPLC.
To utilize a deep-UV light-emitting diode (LED) as a cost-effective radiation source.
To assess the detector's performance for analyzing specific organic compounds.

Main Methods:

A flow-through detector was engineered using a 255 nm deep-UV LED and a UV-sensitive photodiode.
Minimal electronic components, including an operational amplifier, were employed for simplicity and cost-effectiveness.
Compounds (nitrobenzene, benzoic acid, methyl benzoate) were separated using gradient elution HPLC with an acetonitrile/water mobile phase and a specific buffer.

Main Results:

Excellent linearity was achieved with coefficients of determination (r²) of 0.9945, 0.9972, and 0.9996 for quadratic fits.
Low relative standard deviations (n=7) for peak areas were recorded: 0.35% (nitrobenzene), 0.27% (benzoic acid), and 0.83% (methyl benzoate).
Low limits of detection were determined: 750 ng/mL (nitrobenzene), 5.8 µg/mL (benzoic acid), and 12 µg/mL (methyl benzoate).

Conclusions:

The developed deep-UV LED-based HPLC detector is a sensitive, reliable, and cost-effective alternative.
The detector exhibits high performance suitable for quantitative analysis of various organic compounds.
This novel design offers a practical solution for enhancing HPLC capabilities in analytical laboratories.