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Electrospray Ionization (ESI) Mass Spectrometry

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Updated: Jul 6, 2026

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Published on: July 12, 2013

Surface effects and electrochemical cell capacitance in desorption electrospray ionization.

Michael Volný¹, Andre Venter, Scott A Smith

¹Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA.

|March 28, 2008

Summary

Desorption electrospray ionization (DESI) experiments reveal the source acts as a capacitor, influenced by surface properties. Different materials impact charge transfer and analyte oxidation, suggesting super-hydrophobic surfaces are promising substrates.

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In Situ SIMS and IR Spectroscopy of Well-defined Surfaces Prepared by Soft Landing of Mass-selected Ions

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Last Updated: Jul 6, 2026

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Published on: July 12, 2013

In Situ SIMS and IR Spectroscopy of Well-defined Surfaces Prepared by Soft Landing of Mass-selected Ions

In Situ SIMS and IR Spectroscopy of Well-defined Surfaces Prepared by Soft Landing of Mass-selected Ions

Published on: June 16, 2014

Area of Science:

Analytical Chemistry
Surface Science
Mass Spectrometry

Background:

Desorption electrospray ionization (DESI) is a widely used ambient ionization technique.
Understanding the fundamental electrical behavior of the DESI source is crucial for optimizing its performance.
The role of surfaces in DESI experiments is complex and not fully elucidated.

Purpose of the Study:

To investigate the electrical characteristics of the DESI source.
To determine the influence of different surfaces on DESI performance and charge transfer.
To explore the potential of novel surface materials for enhanced DESI analysis.

Main Methods:

Time-resolved current measurements during DESI experiments.
Analysis of charging and discharging behavior with various surfaces.
Correlation of surface properties (e.g., wettability, critical surface tension) with DESI performance.

Main Results:

The DESI source exhibits capacitive behavior, with charging and discharging dynamics observed.
Surface material significantly affects steady-state current and capacitor time constants.
PTFE surfaces demonstrated the longest time constants and highest currents.
Surface energy and wettability correlate with droplet behavior and DESI performance.
Analyte electrochemical oxidation was observed due to surface charge development.

Conclusions:

The DESI source functions as a DC capacitor, with surfaces acting as active electrical elements.
Surface properties, particularly surface energy, are critical for charge transfer in DESI.
Super-hydrophobic materials show potential as superior DESI substrates.
Surface charge can induce electrochemical reactions in analytes during DESI-MS.