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Updated: Jul 6, 2026
Fabrication and Characterization of Layer-By-Layer Janus Base Nano-Matrix to Promote Cartilage Regeneration
Published on: July 6, 2022
[Preliminary study of BMSCs seeded into collagen I-glycosaminoglycan matrices induced toward cartilage]
Wei Hu1, Zhou Xiang, Xueli Zhang
1Department of Orthopaedics, West China Hospital, Sichuan University, Chengdu Sichuan, 610041, P.R. China.
Objective:
To investigate the possibility of BMSCs seeded into collagen I -glycosaminoglycan (CG) matrices to form the tissue engineered cartilage through chondrocyte inducing culture.
Methods:
Bone marrow aspirate of dogs was cultured and expanded to the 3rd passage. BMSCs were harvested and seeded into the dehydrothemal treatment (DHT) cross-linked CG matrices at 1 x 10(6) cells per 9 mm diameter sample. The samples were divided into experimental group and control group. In the experimental group, chondrogenic differentiation was achieved by the induction media for 2 weeks. Medium was changed every other day in both experimental group and control group. The formation of cartilage was assessed by HE staining and collagen II immunohistochemical staining.
Results:
The examinations under the inverted phase contrast microscope indicated the 2nd and 3rd passage BMSCs had the similar morphology. HE staining showed the BMSCs in the experimental group appeared polygon or irregular morphology in the CG matrices, while BMSCs in the control group appeared fibroblast-like spindle or round morphology in the CG matrices. Extracellular matrix could be found around cells in the experimental group. Two weeks after seeded, the cells grew in the CG matrices, and positive collagen II staining appeared around the cells in the experimental group. There was no positive collagen II staining appeared in the control group.
Conclusion:
It is demonstrated that BMSCs seeded CG matrices can be induced toward cartilage by induction media.
