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Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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Related Experiment Video

Updated: Jul 6, 2026

Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1β using a Whole Blood Stimulation Assay

Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1β using a Whole Blood Stimulation Assay

Published on: March 1, 2011

A microchip-based assay for interleukin-6.

Nicolaos Christodoulides¹, Priya Dharshan, Jorge Wong

¹Department of Chemistry and Biochemistry, University of Texas at Austin, USA.

Methods in Molecular Biology (Clifton, N.J.)

|March 28, 2008

Summary

This summary is machine-generated.

The electronic taste chip (ETC) assay system uses bead-based immunoassays on a microchip for detecting analytes. This technology enables precise measurement of biomarkers like interleukin-6 in complex samples.

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Area of Science:

Biotechnology
Analytical Chemistry
Microfluidics

Background:

The electronic taste chip (ETC) assay system is a novel lab-on-a-chip technology.
It utilizes a microchip platform for performing bead-based immunoassays.
Each bead acts as an independent microreactor, with antibody specificity determining analyte detection.

Purpose of the Study:

To describe the application of the ETC system.
To demonstrate the detection and measurement of interleukin-6 (IL-6).

Main Methods:

Bead-based immunoassays performed on a microchip platform within a flow cell.
Utilizes optically transparent polymethylmethacrylate inserts and a metal casing.
Captures and analyzes fluorescent bead images using a digital video chip for quantitation.

Main Results:

The ETC system facilitates the detection of analytes in complex fluids.
The system allows for the quantitation of specific biomarkers.
Successful application for the detection and measurement of IL-6 is described.

Conclusions:

The ETC assay system offers a microfluidic approach for sensitive analyte detection.
This technology enables the quantitation of biomarkers like IL-6 in complex biological samples.
The system's design integrates microreactors, reagent delivery, and digital imaging for efficient analysis.