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Published on: February 1, 2011
Dual fingerprint reagents with enhanced sensitivity: 5-methoxy- and 5-methylthioninhydrin
Joseph Almog1, Asne Klein, Inbal Davidi
1Casali Institute of Applied Chemistry, The Hebrew University of Jerusalem, Jerusalem, Israel. almog@vms.huji.ac.il
Abstract:
"Dual fingerprint reagents" are chemical formulations which produce with latent fingerprints in a single step, impressions that are both colored and fluorescent. Pre-mixed solutions of the two commercially available ninhydrin analogues, 5-methoxyninhydrin (MN) and 5-methylthioninhydrin (MTN) with zinc or cadmium salts, are true dual reagents. They are much more sensitive than the parent dual reagent, ninhydrin/ZnCl(2). The main advantage of the new formulations is that they can be used at room temperature, with no need to cool the sample to liquid nitrogen temperature. At 0.05% concentration, which is 10-fold lower than the common ninhydrin working solution, MTN/ZnCl(2) is as sensitive as DFO in the fluorescence mode and considerably more sensitive in the color mode. MTN is also slightly cheaper than DFO.

