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Updated: Jul 6, 2026

GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil
Published on: May 19, 2012
Using ninhydrin to detect gravesoil
David O Carter1, David Yellowlees, Mark Tibbett
1School of Pharmacy and Molecular Sciences, James Cook University, Townsville, QLD, Australia. dcarter2@unl.edu
Locating buried remains is crucial in death investigations. Research shows cadaver burial significantly increases soil ninhydrin reactive nitrogen (NRN), a potential indicator for identifying clandestine graves.
Area of Science:
- Forensic Science
- Soil Chemistry
- Decomposition Studies
Background:
- Death scene investigations can be hampered by unknown body locations.
- Locating remains or decomposition sites is essential for evidence recovery.
Purpose of the Study:
- To test the hypothesis that mammalian cadaver burial releases ninhydrin reactive nitrogen (NRN) into soil.
- To evaluate NRN as a potential tool for identifying clandestine graves.
Main Methods:
- Juvenile rat cadavers were buried in three Australian tropical savanna soil types.
- Decomposition occurred over 28 days.
- Soil samples were collected and analyzed for NRN concentration.
Main Results:
- Cadaver burial led to an approximate doubling of soil NRN levels (mean increase of 1.7 ± 0.1).
- NRN concentration in soil significantly increased post-burial.
Conclusions:
- The release of NRN into soil following cadaver burial shows potential as a presumptive test for clandestine graves.
- Further research could enhance the application of NRN analysis in forensic investigations.
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