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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jul 6, 2026

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
12:24

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

Published on: July 21, 2014

Affinity-based biosensors as promising tools for gene doping detection.

Maria Minunni1, Simona Scarano, Marco Mascini

  • 1Department of Chemistry, University of Florence, Sesto Fiorentino (Firenze), Italy. maria.minunni@unifi.it

Trends in Biotechnology
|March 28, 2008
PubMed
Summary
This summary is machine-generated.

Gene doping, a misuse of gene therapy, poses detection challenges in sports. This article explores bioanalytical methods and affinity sensing for preventing and detecting this new doping form.

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Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

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Related Experiment Videos

Last Updated: Jul 6, 2026

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
12:24

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

Published on: July 21, 2014

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
08:22

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

Area of Science:

  • Bioanalytical Chemistry
  • Sports Science
  • Genetics

Background:

  • Gene doping, utilizing gene therapy, is a growing concern in anti-doping efforts.
  • Current methods struggle to detect gene doping, raising fears of its undetectability.

Purpose of the Study:

  • To discuss the challenges and potential solutions for detecting gene doping.
  • To explore innovative bioanalytical approaches for anti-doping control.

Main Methods:

  • Review of gene doping aspects, including target analytes and sampling strategies.
  • Discussion of affinity sensing applications in anti-doping.

Main Results:

  • Identification of key challenges in gene doping detection.
  • Exploration of affinity sensing as a potential detection tool.

Conclusions:

  • Innovative bioanalytical methods are crucial for combating gene doping.
  • Affinity sensing shows promise for future anti-doping strategies.