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Isolation of CD146+ Resident Lung Mesenchymal Stromal Cells from Rat Lungs
09:47

Isolation of CD146+ Resident Lung Mesenchymal Stromal Cells from Rat Lungs

Published on: June 17, 2016

Adult lung side population cells have mesenchymal stem cell potential

J Martin1, K Helm, P Ruegg

  • 1Division of Cardiology, Cardiovascular Pulmonary Laboratory, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA.

Cytotherapy
|March 28, 2008
PubMed
Abstract

Insights

Adult lung side population cells are a source of mesenchymal stem cells (MSC), capable of differentiating into multiple cell types. This discovery offers potential for new lung disease therapies and drug interventions.

Area of Science:

  • Pulmonary Medicine
  • Stem Cell Biology
  • Regenerative Medicine

Background:

  • Stem cell therapy for lung diseases is challenging due to the diversity of lung cells and the lack of a common stem cell.
  • Mesenchymal stem cells (MSC) reside in various adult tissues, including the lung.
  • Identifying lung stem cell behavior in disease is crucial for developing effective therapies.

Purpose of the Study:

  • To confirm that non-hematopoietic CD45 negative lung side population (SP) cells contain MSC.
  • To analyze the differentiation potential, chromosomal stability, and telomerase activity of these lung MSC.
  • To establish the utility of lung SP cells as a source of MSC for potential therapeutic applications.

Main Methods:

  • Flow cytometry was used to characterize MSC markers in mouse CD45 negative lung SP cells.
  • Single-cell clones were analyzed for their differentiation potential into chondrocytes, adipocytes, and osteocytes.
  • Karyotype analysis and quantitative polymerase chain reaction assessed chromosomal stability and telomerase activity.

Main Results:

  • CD45 negative lung SP cells expressed key MSC markers and lacked hematopoietic markers.
  • Single-cell clones demonstrated multilineage differentiation potential into chondrocytes, adipocytes, and osteocytes.
  • Cultured and clonal lung SP cells exhibited normal chromosomal structures and high telomerase activity.

Conclusions:

  • Adult CD45 negative lung SP cells are a confirmed source of MSC.
  • This research clarifies the role of tissue-specific lung stem cells in disease.
  • The findings support the potential application of these MSC in lung disease treatments.

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