Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Bacterial Transformation

Bacterial Transformation

In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...

Bacterial Transformation

Bacterial Transformation

In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...

Plasmids

Plasmids

Plasmids are extrachromosomal DNA molecules found in bacteria, archaea, and some eukaryotic microbes like yeast. These small, circular DNA structures typically contain fewer than 30 genes, although some may exist linearly. Plasmids vary in their number within a cell, known as copy number. Single-copy plasmids are present in one copy per cell and multi-copy plasmids are present in multiple copies, reaching over 100 copies per cell.Plasmids usually replicate independently of the chromosomal DNA...

Antibiotic Selection

Antibiotic Selection

DNA Bacteriophages

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...

Recombinant DNA

Recombinant DNA

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Changes in potential pathogenicity-associated proteins of <i>Helicobacter cinaedi</i> upon infection of macrophage cells.

Frontiers in microbiology·2025

Same author

Evaluation of the Biological Properties of the Bacteriodaceae Family Using the Caco-2 Cell Line.

Bulletin of experimental biology and medicine·2024

Same author

Therapeutic Effects of Bacteroides fragilis Vesicles in a Model of Chemically Induced Colitis in Rats.

Bulletin of experimental biology and medicine·2024

Same author

Methodology for Integral Study of Antagonistic Activity of Normal Nasal Microbiota to Select Potential Probiotics Efficient in Eradication of Staphylococcus aureus.

Bulletin of experimental biology and medicine·2023

Same author

Metagenomic assembled plasmids of the human microbiome vary across disease cohorts.

Scientific reports·2022

Same author

[Dynamic11C-methionine PET/CT in differential diagnosis of brain gliomas].

Zhurnal voprosy neirokhirurgii imeni N. N. Burdenko·2021

Search research articles

Related Experiment Video

Updated: Jul 6, 2026

Using Coculture to Detect Chemically Mediated Interspecies Interactions

Using Coculture to Detect Chemically Mediated Interspecies Interactions

Published on: October 31, 2013

[Bifidobacterial plasmids and their application to genetic engineering].

B A Efimov, A N Shkoporov, E V Khokhlova

Vestnik Rossiiskoi Akademii Meditsinskikh Nauk

|March 29, 2008

Summary

Bifidobacterium are vital for gut health but poorly understood genetically. This review covers bifidobacterial plasmids and genetic engineering, essential for developing new probiotics and understanding their health benefits.

More Related Videos

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Published on: March 16, 2012

Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria

Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria

Published on: January 25, 2019

Related Experiment Videos

Last Updated: Jul 6, 2026

Using Coculture to Detect Chemically Mediated Interspecies Interactions

Using Coculture to Detect Chemically Mediated Interspecies Interactions

Published on: October 31, 2013

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Published on: March 16, 2012

Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria

Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria

Published on: January 25, 2019

Area of Science:

Microbiology
Molecular Biology
Genetics

Context:

Bifidobacterium species are crucial for maintaining intestinal homeostasis and overall health.
Despite their importance, the molecular biology and genetics of Bifidobacterium remain underexplored.
Genetic manipulation tools are needed to fully understand their health-promoting mechanisms.

Purpose:

To review existing research on bifidobacterial plasmids and genetic engineering.
To highlight the importance of small plasmids for constructing cloning vectors.
To discuss the current trends and future prospects in the molecular genetics of Bifidobacterium.

Summary:

This review examines pioneering studies on bifidobacterial plasmids and genetic engineering.
It emphasizes the need for genetic tools to engineer Bifidobacterium for therapeutic applications.
The isolation and characterization of small bifidobacterial plasmids are presented as key steps for vector development.

Impact:

Facilitates the development of genetically engineered probiotic Bifidobacterium with enhanced therapeutic properties.
Provides a foundation for future research into the molecular mechanisms underlying Bifidobacterium's health benefits.
Advances the field of microbial genetics, enabling deeper insights into gut microbiome functions.