Related Experiment Video
Updated: Jul 6, 2026

Analysis of Simian Immunodeficiency Virus-specific CD8+ T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining
Published on: December 23, 2016
[Phylogenetic analysis of the mycoplasma isolated from the Javanese macaque (M. fascicularis)]
Abstract:
The nucleotide sequence of the fragment of 16S-aRNA of mycoplasma was determined. The fragment was identified using the PCR method in the urogenital scrape of the Javanese macaque (M. fascicularis). The sequenced fragment of mycoplasma of M. fascicularis was compared to well-known sequences of mycoplasma of mammals. The results of our comparative and phylogenetic analyses of the sequenced DNA fragment revealed that the mycoplasma belonged to the M. primatum species and fell within the same cluster as M. hominis. The mycoplasma M. primatum was for the first time observed in the monkeys M. fascicularis. The pathogenicity of the mycoplasma species with respect to monkeys is being studied.
Insights
Mycoplasma primatum was identified in Javanese macaques using PCR. This marks the first observation of this mycoplasma species in monkeys, with ongoing studies into its pathogenicity.
Area of Science:
- Microbiology
- Genomics
- Primate Health
Context:
- Mycoplasma species are common pathogens in various hosts, including primates.
- Javanese macaques (M. fascicularis) are susceptible to various infections.
- The urogenital tract is a common site for mycoplasma colonization.
Purpose:
- To identify and characterize mycoplasma species in the urogenital tract of Javanese macaques.
- To compare the obtained nucleotide sequence with known mycoplasma sequences.
- To determine the phylogenetic relationship of the identified mycoplasma.
Summary:
- A fragment of 16S-aRNA from mycoplasma was sequenced from a Javanese macaque urogenital sample using PCR.
- Phylogenetic analysis identified the mycoplasma as Mycoplasma primatum, closely related to Mycoplasma hominis.
- This is the first documented instance of Mycoplasma primatum in M. fascicularis.
Impact:
- Establishes the presence of Mycoplasma primatum in a non-human primate host.
- Provides a basis for further research into mycoplasma infections in macaques.
- Informs veterinary diagnostics and management strategies for primate health.
Related Concept Videos
Microbial Phylogeny
Phylogeny
Phylogenetic Species Concept in Microbiology
Modern Molecular Taxonomy
Phylogenetic Trees
Phylogenetic Trees
