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Updated: Jul 6, 2026

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone
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Multiplexed proteomic sample preconcentration device using surface-patterned ion-selective membrane.

Jeong Hoon Lee1, Yong-Ak Song, Jongyoon Han

  • 1Department of Electrical Engineering and Computer Science, Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.

Lab on a Chip
|March 29, 2008
PubMed
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We developed a novel method for creating high-throughput protein preconcentrators using poly(dimethylsiloxane) (PDMS) microfluidic chips. This technique enables rapid prototyping and achieves high concentration factors, enhancing detection sensitivity for mass spectrometry.

Area of Science:

  • Microfluidics
  • Biotechnology
  • Analytical Chemistry

Background:

  • Protein preconcentration is crucial for enhancing the sensitivity of various analytical techniques.
  • Existing methods for fabricating microfluidic preconcentrators can be complex and time-consuming.
  • There is a need for rapid and efficient methods to produce high-throughput preconcentrators.

Purpose of the Study:

  • To develop a new, simplified method for fabricating high-throughput protein preconcentrators in a poly(dimethylsiloxane) (PDMS) microfluidic chip format.
  • To demonstrate the effectiveness of the developed preconcentrator in achieving high concentration factors.
  • To explore the potential for integrating these preconcentrators with detectors like mass spectrometers.

Main Methods:

  • Fabrication of a submicron thick ion-selective membrane on a glass substrate using standard patterning techniques.

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Last Updated: Jul 6, 2026

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone
08:06

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Published on: February 23, 2017

Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes
05:26

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Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

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  • Integration of the ion-selective membrane into a PDMS microfluidic device via plasma-bonding.
  • Rapid prototyping of the PDMS preconcentrator without complex microfabrication processes.

    • Main Results:

    • Achieved a high concentration factor of approximately 10^4 within 5 minutes.
    • Demonstrated the ability to fabricate parallel arrays of preconcentrators for increased sample volume.
    • Successfully integrated the ion-selective membrane into the PDMS microfluidic device.

    Conclusions:

    • The developed printing method offers a rapid and straightforward approach for fabricating high-throughput protein preconcentrators.
    • The high concentration factors achieved make this preconcentrator a valuable tool for enhancing signal detection.
    • The parallel array capability and ease of integration facilitate its application in various analytical systems, including mass spectrometry.