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Adult Stem Cells

Adult Stem Cells

Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously renew...

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Growing Neural Stem Cells from Conventional and Nonconventional Regions of the Adult Rodent Brain

Growing Neural Stem Cells from Conventional and Nonconventional Regions of the Adult Rodent Brain

Published on: November 18, 2013

Adult neural stem cells.

Rossella Galli¹, Angela Gritti, Angelo L Vescovi

¹Stem Cell Research Institute (HSR-SCRI), DIBI-H.S. Raffaele, Milan, Italy.

Methods in Molecular Biology (Clifton, N.J.)

|March 29, 2008

Summary

This summary is machine-generated.

Neural stem cells (NSCs) can be cultured in vitro using the NeuroSphere assay, enabling the expansion of stable NSC lines from various neural tissues. This method isolates proliferating NSCs by using growth factors, eliminating differentiated cells.

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Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord

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Published on: May 14, 2015

Area of Science:

Neuroscience
Stem Cell Biology

Background:

Neural stem cells (NSCs) are present in the mature central nervous system (CNS).
NSCs reside in specific niches within the CNS.

Purpose of the Study:

To describe a method for establishing long-term expanding neural stem cell lines in vitro.
To demonstrate the utility of the NeuroSphere assay for NSC culture.

Main Methods:

Utilizing the NeuroSphere assay for neural stem cell culture.
Continuous exposure of neural cells to mitogens like epidermal growth factor and fibroblast growth factor-2.
Selective proliferation of NSCs and undifferentiated progenitors, eliminating committed cells.

Main Results:

Successful establishment of in vitro NSC cultures.
Generation of stable, long-term expanding NSC lines.
Application of the method to diverse neural tissues, including adult rodent CNS and human brain tumor specimens.

Conclusions:

The NeuroSphere assay is an effective method for culturing and expanding neural stem cells.
This technique allows for the derivation of stable NSC lines from various sources for research purposes.