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Updated: Jul 6, 2026

Classification of Neural Stem Cell Activation State In Vitro Using Autofluorescence

Classification of Neural Stem Cell Activation State In Vitro Using Autofluorescence

Published on: April 12, 2024

Measuring apoptosis in neural stem cells.

Brett T Lund¹, Eve E Kelland

¹Department of Neurology, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|March 29, 2008

Summary

This summary is machine-generated.

Human embryonic stem cells (hESCs) show therapeutic potential for central nervous system (CNS) diseases. Protocols were developed to assess apoptosis in hESCs, crucial for successful CNS stem cell therapy.

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Area of Science:

Neuroscience
Stem Cell Biology
Cell Death Research

Background:

Apoptotic events are common in central nervous system (CNS) damage and disease.
Human embryonic stem cells (hESCs) are potential therapeutics for CNS disorders.
Stem cell therapy success hinges on cell survival and resistance to apoptosis post-transplantation.

Purpose of the Study:

To describe protocols for assessing apoptosis in human embryonic stem cells (hESCs) and their progeny.
To address the limited methods for evaluating apoptosis in adherent stem cell cultures.

Main Methods:

Development and application of specific protocols to detect apoptosis.
Assessment of apoptosis in adherent cultures of hESCs and derived cells.

Main Results:

Established protocols effectively assess apoptosis in hESCs and their progeny.
Demonstrated methods for evaluating the survival capacity of stem cells in CNS-relevant contexts.

Conclusions:

The described protocols provide a means to evaluate hESC apoptosis, vital for CNS regenerative medicine.
Accurate apoptosis assessment is critical for advancing stem cell-based therapies for neurological conditions.