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Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Small interfering RNAs (siRNA)02:30

Small interfering RNAs (siRNA)

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

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Related Experiment Video

Updated: Jul 6, 2026

In ovo Electroporation of miRNA-based Plasmids in the Developing Neural Tube and Assessment of Phenotypes by DiI Injection in Open-book Preparations
12:41

In ovo Electroporation of miRNA-based Plasmids in the Developing Neural Tube and Assessment of Phenotypes by DiI Injection in Open-book Preparations

Published on: October 16, 2012

RNA interference using a plasmid construct expressing short-hairpin RNA.

Serene R Lai1, Lucy G Andrews, Trygve O Tollefsbol

  • 1Department of Biology, University of Alabama Birmingham, Birmingham, AL, USA.

Methods in Molecular Biology (Clifton, N.J.)
|March 29, 2008
PubMed
Summary
This summary is machine-generated.

This study details constructing a plasmid for RNA interference (RNAi) to target the human telomerase reverse transcriptase (hTERT) gene. This method offers a non-viral alternative for long-term gene knockdown in research and potential gene therapy applications.

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DNA Vector-based RNA Interference to Study Gene Function in Cancer
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DNA Vector-based RNA Interference to Study Gene Function in Cancer

Published on: June 4, 2012

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Last Updated: Jul 6, 2026

In ovo Electroporation of miRNA-based Plasmids in the Developing Neural Tube and Assessment of Phenotypes by DiI Injection in Open-book Preparations
12:41

In ovo Electroporation of miRNA-based Plasmids in the Developing Neural Tube and Assessment of Phenotypes by DiI Injection in Open-book Preparations

Published on: October 16, 2012

DNA Vector-based RNA Interference to Study Gene Function in Cancer
13:10

DNA Vector-based RNA Interference to Study Gene Function in Cancer

Published on: June 4, 2012

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Biotechnology

Background:

  • RNA interference (RNAi) is a key technique for gene targeting in modern research and holds promise for clinical applications.
  • Plasmid-based expression of short-hairpin RNAs (shRNAs) provides a method for gene knockdown, serving as an alternative to viral vectors.
  • Plasmid vectors enable sustained gene knockdown without the necessity of creating knockout genotypes.

Purpose of the Study:

  • To describe the methodology for creating a plasmid construct for RNAi-mediated gene targeting.
  • To specifically target the human telomerase reverse transcriptase (hTERT) gene using this plasmid system.

Main Methods:

  • Utilized the Ambion pSilencer system for plasmid construction.
  • Designed shRNAs to target the hTERT gene transcript.
  • Exogenously expressed shRNAs from a plasmid vector.

Main Results:

  • Successfully constructed a plasmid capable of targeting hTERT via RNAi.
  • Demonstrated the feasibility of using plasmid-based shRNA expression for gene knockdown.

Conclusions:

  • Plasmid-based RNAi is a viable strategy for gene targeting and knockdown.
  • This approach offers a non-viral, long-term gene silencing method with potential therapeutic implications.