Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Lytic Cycle of Bacteriophages01:30

Lytic Cycle of Bacteriophages

Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the lytic replication...
Viral Replication: Lytic Cycle01:20

Viral Replication: Lytic Cycle

Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...
Biological Methods for Microbial Control01:28

Biological Methods for Microbial Control

Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
Lysosomes01:31

Lysosomes

Lysosomes are membrane-enclosed spherical sacs derived from the Golgi apparatus. The most important function of the lysosome is degrading macromolecules and biological polymers that are released during membrane trafficking events such as the secretory, endocytic, autophagic, and phagocytic pathways. The degradation is carried out by several hydrolytic enzymes active in an acidic environment of the lysosomal lumen. These acid hydrolases are involved in cellular processes such as cell signaling,...
Transformation01:26

Transformation

Microbial communities are dynamic environments where cell lysis releases free DNA into the surroundings. Other cells can take up this extracellular DNA through a process known as transformation.When a cell incorporates this foreign DNA into its genome, resulting in genetic modification, the process is known as transformation. Cells capable of this process are termed competent. Competence can be natural, as observed in certain bacteria and archaea, or artificially induced in the...
Tissue Homogenization and Cell Lysis01:32

Tissue Homogenization and Cell Lysis

Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods.
Mechanical methods of tissue homogenization
These methods rely on applying external physical force to disrupt...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Xylan extraction from hardwoods by alkaline pretreatment for xylooligosaccharide production: A detailed fractionation analysis.

Carbohydrate polymers·2023
Same author

openPFGE: An open source and low cost pulsed-field gel electrophoresis equipment.

HardwareX·2022
Same author

Iron-meditated fungal starvation by lupine rhizosphere-associated and extremotolerant Streptomyces sp. S29 desferrioxamine production.

Molecular omics·2020
Same author

Xylooligosaccharides from lignocellulosic biomass: A comprehensive review.

Carbohydrate polymers·2020
Same author

Saccharification of Brown Macroalgae Using an Arsenal of Recombinant Alginate Lyases: Potential Application in the Biorefinery Process.

Journal of microbiology and biotechnology·2018
Same author

The effect of a lytic polysaccharide monooxygenase and a xylanase from Gloeophyllum trabeum on the enzymatic hydrolysis of lignocellulosic residues using a commercial cellulase.

Enzyme and microbial technology·2018

Related Experiment Video

Updated: Jul 6, 2026

Budding Yeast Protein Extraction and Purification for the Study of Function, Interactions, and Post-translational Modifications
09:22

Budding Yeast Protein Extraction and Purification for the Study of Function, Interactions, and Post-translational Modifications

Published on: October 30, 2013

Bacteria and yeast cell disruption using lytic enzymes.

Oriana Salazar1

  • 1University of Chile, Santiago, Chile.

Methods in Molecular Biology (Clifton, N.J.)
|March 29, 2008
PubMed
Summary

Enzymatic protein extraction offers advantages over mechanical methods. This chapter details protocols for bacteria and yeast, adaptable for high-throughput proteomic screening.

Area of Science:

  • Biochemistry and Molecular Biology
  • Microbiology
  • Biotechnology

Background:

  • Mechanical cell disruption for protein extraction presents technical challenges.
  • Enzymatic methods offer a more convenient alternative for releasing intracellular proteins.
  • Efficient protein extraction is crucial for various biological applications, including proteomics.

Purpose of the Study:

  • To present enzymatic protocols for protein extraction from bacteria and Saccharomyces cerevisiae.
  • To adapt these protocols for high-throughput screening and proteomic applications.
  • To demonstrate the versatility of enzymatic methods for different microbial systems.

Main Methods:

  • Utilized lytic enzymes for the disruption of bacterial and yeast cell walls.

More Related Videos

Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases
07:47

Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases

Published on: January 1, 2016

Preparation of Cell Extracts by Cryogrinding in an Automated Freezer Mill
14:00

Preparation of Cell Extracts by Cryogrinding in an Automated Freezer Mill

Published on: January 29, 2021

Related Experiment Videos

Last Updated: Jul 6, 2026

Budding Yeast Protein Extraction and Purification for the Study of Function, Interactions, and Post-translational Modifications
09:22

Budding Yeast Protein Extraction and Purification for the Study of Function, Interactions, and Post-translational Modifications

Published on: October 30, 2013

Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases
07:47

Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases

Published on: January 1, 2016

Preparation of Cell Extracts by Cryogrinding in an Automated Freezer Mill
14:00

Preparation of Cell Extracts by Cryogrinding in an Automated Freezer Mill

Published on: January 29, 2021

  • Developed and optimized protein extraction protocols based on enzymatic activity.
  • Adapted the yeast protocol for a microtiter plate format suitable for large-scale screening.

    • Main Results:

    • Successfully established enzymatic protocols for efficient protein extraction from both bacteria and yeast.
    • Demonstrated the applicability of the adapted yeast protocol for proteomic analyses.
    • Validated the methodology's potential for high-throughput screening of genetic libraries.

    Conclusions:

    • Enzymatic protein extraction is a viable and advantageous alternative to mechanical disruption.
    • The presented protocols are effective for diverse microbial species, including bacteria and yeast.
    • The microtiter plate adaptation facilitates high-throughput proteomic studies and genetic variant screening.