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Related Experiment Video

Updated: Jul 6, 2026

Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes

Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes

Published on: November 26, 2008

Radiolabeling for two-dimensional gel analysis.

Hélian Boucherie¹, Aurélie Massoni, Christelle Monribot-Espagne

¹Centre National de la Recherche Scientifique, Bordeaux, France.

Methods in Molecular Biology (Clifton, N.J.)

|March 29, 2008

Summary

This study details radiolabeling protocols for sensitive protein detection using radioactive amino acids. It enables protein visualization and comparison via two-dimensional gel electrophoresis.

Area of Science:

Biochemistry
Molecular Biology
Proteomics

Background:

Radiolabeling offers high sensitivity for protein detection.
Incorporation of radioactive amino acids is a key method.
It is ideal for visualizing proteins on 2-D gels.

Purpose of the Study:

To present protocols for in vivo radiolabeling.
To optimize labeling conditions for protein analysis.
To facilitate comparative proteomic studies using 2-D gel electrophoresis.

Main Methods:

In vivo radiolabeling using radioactive amino acids.
Optimization of labeling conditions.
Two-dimensional (2-D) gel electrophoresis for protein separation and visualization.

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From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia

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Last Updated: Jul 6, 2026

Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes

Selective Labelling of Cell-surface Proteins using CyDye DIGE Fluor Minimal Dyes

Published on: November 26, 2008

Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase

Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase

Published on: February 12, 2019

From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia

From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia

Published on: October 19, 2014

Main Results:

Established protocols for effective protein radiolabeling.
Demonstrated the utility of radiolabeling for protein sample comparison.
Enabled sensitive detection of proteins separated by 2-D gels.

Conclusions:

Radiolabeling is a valuable technique for sensitive protein detection and analysis.
The presented protocols support comparative proteomics using 2-D gel electrophoresis.
This method enhances protein visualization and quantification.