Related Experiment Video

Updated: Jul 6, 2026

Apoplast-Extraction Based Method to Improve the Purity of Plant Produced Recombinant Protein
05:33

Apoplast-Extraction Based Method to Improve the Purity of Plant Produced Recombinant Protein

Published on: July 5, 2024

Protein extraction from green plant tissue

Ragnar Flengsrud1

  • 1Norwegian University of Life Sciences, As, Norway.

Methods in Molecular Biology (Clifton, N.J.)
|March 29, 2008
PubMed

Abstract:

A method for preparation of protein from green plant tissue for two-dimensional electrophoresis is described. The method is demonstrated on barley leaves, potato leaves and spruce needles and appears to overcome the obstacles inherent in green plants to proteomic analysis. The yield and the representation of proteins are discussed.

More Related Videos

Identification of Post-translational Modifications of Plant Protein Complexes
10:07

Identification of Post-translational Modifications of Plant Protein Complexes

Published on: February 22, 2014

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
11:17

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

Published on: June 1, 2017

Related Experiment Videos

Last Updated: Jul 6, 2026

Apoplast-Extraction Based Method to Improve the Purity of Plant Produced Recombinant Protein
05:33

Apoplast-Extraction Based Method to Improve the Purity of Plant Produced Recombinant Protein

Published on: July 5, 2024

Identification of Post-translational Modifications of Plant Protein Complexes
10:07

Identification of Post-translational Modifications of Plant Protein Complexes

Published on: February 22, 2014

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
11:17

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

Published on: June 1, 2017

Related Concept Videos

DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
DNA Isolation01:34

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

Articles linked to this work by shared authors, journal, and citation graph.

Disaccharide analysis of chondroitin and heparin from farmed Atlantic salmon.

Glycoconjugate journal·2016

The binding of pentapeptides to biological and synthetic high affinity heparin.

Bioorganic & medicinal chemistry letters·2015

Affinity chromatography, two-dimensional electrophoresis, adapted immunodepletion and mass spectrometry used for detection of porcine and piscine heparin-binding human plasma proteins.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2013

Antibacterial peptides derived from caprine whey proteins, by digestion with human gastrointestinal juice.

The British journal of nutrition·2011

Purification, characterization and in vivo studies of salmon heparin.

Thrombosis research·2010

Structural characteristic, pH and thermal stabilities of apo and holo forms of caprine and bovine lactoferrins.

Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine·2010

Graph-Based Gene Embedding.

Methods in molecular biology (Clifton, N.J.)·2026

Copy Number Variation Analysis.

Methods in molecular biology (Clifton, N.J.)·2026

AI/ML-Driven Gene Analysis: New Perspectives on Variant Calling in Normal Human Tissue Using scRNA-seq Data.

Methods in molecular biology (Clifton, N.J.)·2026

RNA Structure and Its Function.

Methods in molecular biology (Clifton, N.J.)·2026

Comprehensive Guide to Gene Expression Analysis from Bulk and Single-Nucleus Transcriptomes.

Methods in molecular biology (Clifton, N.J.)·2026

Large Language Models for Non-Coding RNA Biomarker Discovery in Breast Cancer.

Methods in molecular biology (Clifton, N.J.)·2026

Current insights into PHF14: molecular features and functional roles.

Frontiers in molecular biosciences·2026

Integrated metabolomics and transcriptomics reveal that Lolium rigidum ABCC3.2 contributes to multiple herbicide resistance.

Pesticide biochemistry and physiology·2026

A loss-of-function allele fixed during domestication reshapes nectar chemistry, microbial diversity, and pollinator visits.

Current biology : CB·2026

Glucose-TOR-PDCB signaling restricts cell-to-cell communication through callose deposition.

Science advances·2026

A GRX-SOD module maintains superoxide homeostasis and meristem development in maize.

Advanced biotechnology·2026

NtFATB silencing impairs lipid homeostasis and enhances cold sensitivity associated with reduced the ICE-CBF-COR expression in Nicotiana tabacum.

Plant physiology and biochemistry : PPB·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies
Jove
Visualize
Contact Us