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Human kidney hexosaminidase A and hexosaminidase B form a complex
A V Pshezhetsky1, O A Buneeva, V M Dvorkin
1Belozersky Laboratory, Moscow State University, USSR.
European Journal of Biochemistry
|December 5, 1991
Abstract:
The isolation and purification of human kidney hexosaminidases A and B was carried out. Regulation of the supramolecular organization and catalytic activity of hexosaminidases was investigated in the bis(2-ethylhexyl)sulphosuccinate reversed micellar system modeling the enzyme microenvironment in the lysosomes. It was shown that hexosaminidases A and B associate forming 280-300-kDa dimeric complexes under these conditions. At pH 4.75, the hexosaminidases A and B can be isolated from kidney tissue only in the form of this complex.