Related Experiment Video
Updated: Jul 6, 2026

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
Published on: February 18, 2022
Bioinformatics approaches for studying untranslated regions of mRNAs
1Bioinformatics Department, Ramapo College of New Jersey, Mahwah, NJ, USA.
Abstract:
Interactions between RNA-binding proteins and cis-acting elements in the 5'- and 3'-untranslated regions (UTRs) of transcripts are responsible for regulating essential biological activities, such as mRNA localization, mRNA turnover, and translation efficiency. This chapter introduces some of the publicly available free bioinformatics resources, including software tools and databases, which can be used for predicting, mapping, and characterizing regulatory motifs found in the eukaryotic mRNA-untranslated regions.
Related Concept Videos
Ribosome Profiling
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Leaky Scanning
Regulated mRNA Transport
Translational Regulation
Nonsense-mediated mRNA Decay
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
MicroRNAs

