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Related Experiment Video

Updated: Jul 6, 2026

Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells

Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells

Published on: May 12, 2023

Intercellular trafficking of macromolecules during embryogenesis.

Insoon Kim¹, Patricia C Zambryski

¹Department of Biology, Sungshin Women's University, Korea.

Methods in Molecular Biology (Clifton, N.J.)

|March 29, 2008

Summary

This summary is machine-generated.

Plant cell communication via plasmodesmata changes during embryogenesis. A new assay reveals a developmental shift in Arabidopsis at the torpedo stage, restricting transport and aiding mutant screening.

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Published on: August 16, 2021

Area of Science:

Plant biology
Cell biology
Developmental biology

Background:

Plasmodesmata are crucial for intercellular communication and nutrient transport in plants, connecting the cytoplasm of adjacent cells.
Understanding the regulation of plasmodesmata during plant development is essential for comprehending growth and differentiation.

Purpose of the Study:

To develop and utilize a fluorescent tracer-loading assay to monitor cell-to-cell transport through plasmodesmata during plant embryogenesis.
To investigate developmental changes in the size exclusion limit of plasmodesmata in Arabidopsis.
To screen for mutants with altered plasmodesmata function during embryogenesis.

Main Methods:

Development of a simple fluorescent tracer-loading assay.
Monitoring cell-to-cell transport via plasmodesmata during Arabidopsis embryogenesis.
Screening for embryo defective mutants affecting plasmodesmata.

Main Results:

A developmental transition in plasmodesmatal size exclusion limit was identified at the torpedo stage of Arabidopsis embryogenesis.
At the torpedo stage, plasmodesmata are downregulated, permitting the transport of small tracers (approx. 0.5 kDa) but restricting larger ones (approx. 10 kDa).
Identification of embryo defective mutants, termed 'increased size exclusion limit of plasmodesmata' (ise), which exhibit dilated plasmodesmata at the torpedo stage.

Conclusions:

The developed assay effectively monitors plasmodesmata-mediated transport during embryogenesis.
Plasmodesmatal regulation undergoes a significant transition during embryogenesis, impacting intercellular transport.
The identified mutants provide tools to study the genetic control of plasmodesmata function and its role in plant development.