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Updated: Jul 6, 2026
13:08
Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass
Published on: August 10, 2017
Proteomic analysis of the stacked Golgi complex
Mark S Lowenthal1, Kathryn E Howell, Christine C Wu
1Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO, USA.
Methods in Molecular Biology (Clifton, N.J.)
|March 29, 2008
Summary
Researchers isolated Golgi fractions from rat liver and analyzed their proteomes using multidimensional protein identification technology (MudPIT). This provides a molecular framework for understanding the Golgi complex and secretory pathway.
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- Mammalian organelle structure and function are well-studied, but comprehensive proteomes are needed for molecular integration.
- The Golgi complex is a key organelle in the secretory pathway, crucial for protein and lipid modification and sorting.
Purpose of the Study:
- To describe methods for isolating an enriched stacked Golgi fraction (GF) from rat liver.
- To perform shotgun proteomic analysis of the isolated Golgi fraction using MudPIT.
Main Methods:
- Isolation of an enriched stacked Golgi fraction (GF) from rat liver.
- Shotgun proteomic analysis utilizing multidimensional protein identification technology (MudPIT).
Main Results:
- Successful isolation of a purified Golgi fraction.
- Identification of a comprehensive set of proteins within the Golgi complex.
Conclusions:
- The developed methods enable detailed proteomic analysis of the Golgi complex.
- This proteomic data provides a molecular framework for understanding Golgi function within the secretory pathway.
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