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Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice
Published on: August 23, 2007
Intravital two-photon imaging of natural killer cells and dendritic cells in lymph nodes
Susanna Celli1, Béatrice Breart, Philippe Bousso
1Département d'Immunologie, G5 Dynamiques des Réponses Immunes, Equipe Avenir, Institut Pasteur, Paris Cedex, France.
Methods in Molecular Biology (Clifton, N.J.)
|March 29, 2008
Abstract:
Two-photon microscopy makes it possible to image in real-time fluorescently labeled cells located in deep tissue environments. We describe a procedure to visualize the behavior of natural killer (NK) cells and dendritic cells (DC) in the lymph nodes of live, anesthetized mice. Intravital two-photon imaging is a powerful tool to study the migration and cell interactions of immune cells such as NK and DC in physiological settings.
