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An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets
Published on: April 18, 2016
Clinical analysis of dendritic cell subsets: the dendritogram
Anne Hosmalin1, Miriam Lichtner, Stéphanie Louis
1Institut Cochin, Département d'Immunologie, Paris, France.
Methods in Molecular Biology (Clifton, N.J.)
|March 29, 2008
Summary
Reduced numbers of dendritic cells (DCs), including plasmacytoid DCs (pDCs) and myeloid DCs (mDCs), are observed in HIV infection. Lower pDC counts correlate with higher viral load and may predict disease severity or treatment success in various infections.
Area of Science:
- Immunology
- Infectious Diseases
- Cell Biology
Background:
- Dendritic cells (DCs) are essential antigen-presenting cells for adaptive immunity.
- Plasmacytoid DCs (pDCs) are key producers of type I Interferons during infection.
- Reduced circulating myeloid DC (mDC) and pDC numbers are documented in HIV infection.
Purpose of the Study:
- To investigate the clinical utility of DC counts in infectious diseases.
- To explore the correlation between pDC counts and HIV viral load.
- To assess the predictive value of DC counts for disease progression and treatment outcomes.
Main Methods:
- Flow cytometry and rare event analysis were used to quantify pDC counts.
- Correlation analysis was performed between pDC counts and viral load in HIV patients.
- The predictive value of pDC counts was examined in dengue and tuberculosis infections.
Main Results:
- Circulating mDC and pDC numbers are decreased in both primary and chronic HIV infection.
- pDC counts show an inverse correlation with HIV viral load.
- pDC counts may serve as an early indicator of recovery post-antiretroviral therapy and predict better immune control.
- pDC counts may predict disease severity in dengue and treatment success in tuberculosis.
Conclusions:
- DC counts, particularly pDCs, are valuable biomarkers in infectious diseases.
- The "dendritogram" (DC counts) may aid in the clinical assessment of HIV, dengue, and tuberculosis.
- Monitoring DC populations offers insights into immune status and treatment response in various infections.
