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Updated: Jul 6, 2026

AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain
Published on: March 31, 2022
In vitro analysis of translation enhancers
Aurélie M Rakotondrafara1, W Allen Miller
1Molecular Cellular and Developmental Biology, Department of Plant Pathology, Iowa State University, Ames, IA, USA.
Abstract:
The genomes of many plant viruses contain translation-enhancing sequences that allow them to compete successfully with host messenger RNAs for the translation machinery. Identification of translation enhancer elements is valuable, both to gain understanding of virus gene expression control and to apply them as tools for engineering gene expression in plant cells. Here, we describe experiments designed to detect viral elements that enhance translation, focusing on cap-independent translation activity, using a high fidelity cell-free wheat germ translation extract.
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