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In situ detection of plant viruses and virus-specific products

Andrew J Maule1, Zoltán Havelda

  • 1John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK.

Methods in Molecular Biology (Clifton, N.J.)
|March 29, 2008
PubMed

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In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...