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High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
Published on: February 7, 2019
A conserved domain in the transcription factor ITF-2B attenuates its activity
1Department of Medicine II, University of Munich, Marchioninistr. 15, 81377 Munich, Germany. Andreas.Herbst@med.uni-muenchen.de
Abstract:
The immunoglobulin transcription factor-2B (ITF-2B) belongs to the basic helix-loop-helix (bHLH) family of transcription factors. It is ubiquitously expressed and plays a prominent role in the regulation of differentiation processes. Protein sequence alignment of the closely related bHLH transcription factors ITF-2B, HeLa E box protein (HITF4), and the E2A proteins E12 and E47 revealed the presence of a highly conserved protein domain. Functional analysis of this domain demonstrated that it plays an important role in repressing the transcriptional activity of the ITF-2B protein. Moreover, this domain comprises a self-contained transcriptional repressor whose activity depends on specific amino acid residues.
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