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Quantitative liquid chromatography/mass spectrometry for the analysis of microdialysates.

Katrien Lanckmans¹, Sophie Sarre, Ilse Smolders

¹Research Group Experimental Pharmacology, Department of Pharmaceutical Chemistry, Drug Analysis and Drug Information, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium.

|March 29, 2008

Summary

Microdialysis (MD) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) enables sensitive quantification of molecules in biological samples. This review covers LC-MS/MS methods for analyzing endogenous and exogenous compounds in microdialysates.

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Area of Science:

Biochemistry
Analytical Chemistry
Pharmacology

Background:

Microdialysis (MD) is an in vivo technique for sampling extracellular fluid from tissues.
MD yields protein- and cell-free samples suitable for direct analysis.
Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) offers high sensitivity and selectivity for quantifying analytes.

Purpose of the Study:

To review LC-MS/MS methods for quantifying molecules in microdialysates.
To highlight the importance of miniaturization for enhancing analytical sensitivity.
To discuss challenges in quantifying low-concentration analytes in microdialysis samples.

Main Methods:

Review of published LC-MS/MS methods applied to microdialysis samples.
Focus on methods for endogenous (neurotransmitters, peptides) and exogenous (drugs) compounds.
Discussion of analytical technique miniaturization strategies.

Main Results:

LC-MS/MS is a suitable technique for analyzing microdialysates.
Miniaturization of LC-MS/MS is crucial for maximizing sensitivity.
Key challenges include matrix effects, low microdialysis efficiency, and analyte adsorption.

Conclusions:

LC-MS/MS is a powerful tool for analyzing microdialysates.
Optimizing LC-MS/MS miniaturization and addressing analytical challenges are essential for accurate quantification.
This review provides insights into current methodologies and future directions in microdialysis analysis.