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Updated: Jul 6, 2026

Direct Detection of the Acetate-forming Activity of the Enzyme Acetate Kinase
Published on: December 19, 2011
Study on acetate ion recognition and sensing in aqueous media using a novel and simple colorimetric sensor and its
1Department of Chemistry, Nankai University, Weijin Road 94#, Tianjin 300071, PR China.
Abstract:
A new and simple phenylhydrazone-based sensor 1 for acetate has been investigated in DMSO and even in the 95:5 DMSO/H2O (v/v) mixtures. Binding of anions such as AcO-, F- and H2PO4(-) results in a noteworthy change in the visible region of spectrum (approximately 100 nm red shift), which can be detected by the 'naked-eye'. The further insights to the nature of interactions between the sensor 1 and AcO- were investigated by 1H NMR titration experiments in DMSO-d(6). In particular, analytical application and detection of acetate ion have been also studied in the aqueous.
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