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Updated: Jul 6, 2026
Preparation of Naringenin Solution for In Vivo Application
Published on: August 10, 2021
Isolation of the MAO-inhibitor naringenin from Mentha aquatica L
Helle T Olsen1, Gary I Stafford, Johannes van Staden
1Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen, 2 Universitetsparken, 2100 Copenhagen O, Denmark.
Aims Of The Study:
To isolate the compound(s) responsible for the MAO-inhibitory activity.
Materials And Methods:
Six extracts of varying polarity of Mentha aquatica L. were tested in a photometric peroxidase linked MAO bioassay. The 70% ethanol extract had highest inhibitory activity. (S)-Naringenin was isolated from the extract by bioassay guided fractionation on VLC and preparative TLC. The structure of the compound was determined by (1)H, (13)C and (13)C-DEPT NMR and optical rotation.
Results:
The IC(50) values for MAO inhibition by naringenin were 342+/-33 microM for the rat liver mitochondrial fraction, 955+/-129 microM for MAO-A and 288+/-18 microM for MAO-B.
Conclusions:
The content of naringenin in Mentha aquatica might explain its use in traditional medicine for depression-like conditions.
