Related Experiment Video
Updated: Jan 31, 2026

High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay
Published on: January 7, 2019
NPC1L1 and SR-BI are involved in intestinal cholesterol absorption from small-size lipid donors
Ziad Haikal1, Barbara Play, Jean-François Landrier
1INRA, UMR1260 Nutriments Lipidiques et Prévention des Maladies Métaboliques, INSERM, U476, Univ Aix-Marseille 1, Univ Aix-Marseille 2, Faculté de Médecine, IPHM-IFR 125, 27 bld Jean Moulin, 13385 Marseille, France.
Abstract:
In the human intestinal content after a meal, cholesterol is dispersed in a complex mixture of emulsified droplets, vesicles, mixed micelles and precipitated material. The aim of this study was to determine the contribution of the main intestinal cholesterol transporters (NPC1L1, SR-BI) to the absorption processes, using different cholesterol-solubilizing donors. Cholesterol donors prepared with different taurocholate concentrations were added to an apical medium of differentiated TC7/Caco-2 cells. As the taurocholate concentrations increased, cholesterol donor size decreased (from 712 to 7 nm in diameter), which enhanced cholesterol absorption in a dose-dependent manner (38-fold). Two transport processes were observed: (1) absorption from large donors exhibited low-capacity transport with no noticeable transporter contribution; (2) efficient cholesterol absorption occurs from small lipid donors (
Related Concept Videos
Lipid Absorption
These breakdown products bind with bile salts and lecithin to form micelles, which quickly pass between microvilli to come in close contact with the apical...
Glucose Absorption Into the Small Intestine
What are Lipids?
Lipid Digestion
Cholesterol: Significance and Regulation
Considering cholesterol and...
Structure of Lipids

