A simple silica-based method for metagenomic DNA extraction from soil and sediments
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Updated: Jul 6, 2026

Extraction of High Molecular Weight Genomic DNA from Soils and Sediments
Published on: November 10, 2009
R Rojas-Herrera1, J Narváez-Zapata, M Zamudio-Maya
1Universidad Autónoma de Yucatán, Ciudad Industrial, Mérida, Yucatán, Mexico. r.rojas@uady.mx
Abstract:
A new method is described for extraction of metagenomic DNA from soil and sediments which is based on DNA adsorption to silica without the use of phenol, ethanol precipitation or a cesium chloride gradient. High-quality DNA was obtained, and PCR inhibition was overcome by adding bovine serum albumin and adjusting magnesium concentration. By using PCR-DGGE with Firmicutes and lactic acid bacteria-specific primers the extracted metagenomic DNA was shown to contain a mixture of bacterial genomes. This method can be used for screening bacterial diversity in soil and sediment samples.
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