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Related Experiment Video

Updated: Jul 6, 2026

Human Pancreatic Islet Isolation: Part I: Digestion and Collection of Pancreatic Tissue
11:54

Human Pancreatic Islet Isolation: Part I: Digestion and Collection of Pancreatic Tissue

Published on: May 26, 2009

Factors that affect human islet isolation.

Y Sakuma1, C Ricordi, A Miki

  • 1Cell Transplant Center, Diabetes Research Institute, University of Miami Leonard M. Miller School of Medicine, Miami, Florida 33136, USA.

Transplantation Proceedings
|April 1, 2008
PubMed
Summary
This summary is machine-generated.

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Achieving insulin independence in type 1 diabetes requires successful islet isolation. This study found that while donor BMI is important, pancreatic surface integrity and optimized processing are key to improving islet isolation success rates.

Area of Science:

  • Transplantation immunology
  • Endocrinology
  • Surgical research

Background:

  • Type 1 diabetes mellitus (T1DM) treatment often requires islet transplantation.
  • Achieving insulin independence necessitates a high yield of isolated islets (10,000 IEQ/kg recipient weight).
  • Donor characteristics like high body mass index (BMI) and pancreas size impact islet isolation success, but organ donor shortage limits options.

Purpose of the Study:

  • To identify critical factors influencing human islet isolation outcomes.
  • To optimize islet isolation protocols for improved success rates, especially from donors with suboptimal characteristics.

Main Methods:

  • Analysis of 207 islet isolation procedures conducted between 2002 and 2006.
  • Evaluation of donor characteristics, pancreas condition, and processing variables.

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Human Pancreatic Islet Isolation: Part II: Purification and Culture of Human Islets
08:35

Human Pancreatic Islet Isolation: Part II: Purification and Culture of Human Islets

Published on: May 26, 2009

Pancreatic Islet Isolation and Purification from Lewis Rats Using Enzymatic Digestion and Density-Gradient Separation
07:44

Pancreatic Islet Isolation and Purification from Lewis Rats Using Enzymatic Digestion and Density-Gradient Separation

Published on: April 3, 2026

Related Experiment Videos

Last Updated: Jul 6, 2026

Human Pancreatic Islet Isolation: Part I: Digestion and Collection of Pancreatic Tissue
11:54

Human Pancreatic Islet Isolation: Part I: Digestion and Collection of Pancreatic Tissue

Published on: May 26, 2009

Human Pancreatic Islet Isolation: Part II: Purification and Culture of Human Islets
08:35

Human Pancreatic Islet Isolation: Part II: Purification and Culture of Human Islets

Published on: May 26, 2009

Pancreatic Islet Isolation and Purification from Lewis Rats Using Enzymatic Digestion and Density-Gradient Separation
07:44

Pancreatic Islet Isolation and Purification from Lewis Rats Using Enzymatic Digestion and Density-Gradient Separation

Published on: April 3, 2026

  • Statistical analysis to determine significant factors affecting isolation outcome (defined as >3000 IEQ/g pancreas weight).

    • Main Results:

    • Islet isolation success was significantly higher from donors with BMI >30 kg/m2 (P = .002).
    • Pancreatic surface integrity was a significant positive factor (P = .02).
    • Longer digestion times (P = .04) and a higher proportion of trapped islets negatively impacted success rates (P = .004).

    Conclusions:

    • While high BMI donors yield more islets, optimizing factors like pancreatic surface integrity and reducing digestion time are crucial for improving islet isolation success.
    • Enhanced protocols and systematic training for processing teams can increase the number of successful human islet isolations.
    • Focusing on controllable processing variables can mitigate challenges posed by donor organ shortages.