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Updated: Jul 6, 2026
10:23
Absolute Quantification of Plasma MicroRNA Levels in Cynomolgus Monkeys, Using Quantitative Real-time Reverse Transcription PCR
Published on: February 12, 2018
Investigation of cynomolgus monkey complement
1Division of Organ Transplantation, Department of Molecular Therapeutics, Osaka University Graduate School of Medicine, Osaka, Japan.
Transplantation Proceedings
|April 1, 2008
Summary
Human decay accelerating factor (CD55) demonstrates similar complement regulatory function in both human and cynomolgus monkey sera. This finding is crucial for advancing xenotransplantation research using cynomolgus monkeys.
Area of Science:
- Immunology
- Xenotransplantation Research
Background:
- Cynomolgus monkeys are key recipients in xenotransplantation.
- Limited research exists on cynomolgus monkey complement systems.
- Complement regulation is vital for xenograft survival.
Purpose of the Study:
- To investigate the complement regulatory function of human decay accelerating factor (CD55) in cynomolgus monkey serum.
- To compare this function between human and cynomolgus monkey sera.
Main Methods:
- Hemolytic complement titers were measured in cynomolgus monkeys using human-standard methods.
- The regulatory function of human decay accelerating factor (CD55) was assessed on porcine endothelial cells (PECs) in both human and cynomolgus monkey sera.
Main Results:
- Complement titers (CH50, ACH50, C2, C3) were generally high in cynomolgus monkeys, with C4 being an exception.
- Human decay accelerating factor (CD55) exhibited comparable complement regulatory activity in both human and cynomolgus monkey sera when applied to porcine endothelial cells.
Conclusions:
- Human decay accelerating factor (CD55) functions similarly in regulating complement in both human and cynomolgus monkey sera.
- This suggests potential for using human CD55 to mitigate complement-mediated rejection in cynomolgus monkey xenotransplantation models.
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