Search research articles

Search research articles

Related Experiment Video

Updated: Jul 6, 2026

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag

Published on: December 14, 2017

Detection of compounds that rescue Rab1-synuclein toxicity.

James Fleming¹, Tiago F Outeiro, Mark Slack

¹FoldRx Pharmaceuticals, Inc., Cambridge, Massachusetts, USA.

Methods in Enzymology

|April 1, 2008

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The road towards faster development of novel pharmacotherapies for persons with Parkinson's disease.

Journal of Parkinson's disease·2026

Same author

Progression of Dysarthria, Drooling, and Swallowing Disorders in Parkinson's Disease: A 1-Year Prospective Cohort Study.

American journal of speech-language pathology·2026

Same author

Disease modification in advanced Parkinson's disease: a review and roadmap for paving the way for next-generation interventions.

Journal of neural transmission (Vienna, Austria : 1996)·2026

Same author

Lewy Bodies Are Not Associated With Neuronal or Synaptic Loss in Dementia With Lewy Bodies.

Neuropathology and applied neurobiology·2026

Same author

Blm10/PA200-Activated 20S Proteasomes Promote α-Synuclein Degradation and Bypass Proteasome Inhibition in Parkinson's Disease Models.

Aging cell·2026

Same author

Knockout of the LRRK2-counteracting RAB phosphatase PPM1H disrupts axonal autophagy and exacerbates alpha-synuclein aggregation.

Cell reports·2026

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Parkinson's disease (PD) may involve disrupted Rab protein trafficking and alpha-synuclein (asyn) misfolding. Overexpressing Rab proteins can rescue asyn toxicity, suggesting new drug discovery avenues for PD.

Area of Science:

Neuroscience
Molecular Biology
Genetics

Background:

Parkinson's disease (PD) is linked to alpha-synuclein (asyn) misfolding and neuronal death.
Rab-mediated protein trafficking disruptions are implicated in neurodegeneration.
Overexpression of Rab proteins rescues asyn toxicity in various model systems.

Purpose of the Study:

To describe assay formats for identifying small molecules that rescue asyn toxicity in yeast.
To provide methods for high-throughput screening of potential Parkinson's disease therapeutics.

Main Methods:

Yeast-based screening using two distinct assay formats.
Assay 1: 96-well plate format measuring optical density for compound screening.
Assay 2: AlamarBlue dye reduction assay for viable cell measurement in various plate formats (96, 384, 1536-well).

More Related Videos

Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson's Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation

Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson's Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation

Published on: March 21, 2020

A Method to Study α-Synuclein Toxicity and Aggregation Using a Humanized Yeast Model

A Method to Study α-Synuclein Toxicity and Aggregation Using a Humanized Yeast Model

Published on: November 25, 2022

Related Experiment Videos

Last Updated: Jul 6, 2026

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag

Published on: December 14, 2017

Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson's Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation

Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson's Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation

Published on: March 21, 2020

A Method to Study α-Synuclein Toxicity and Aggregation Using a Humanized Yeast Model

A Method to Study α-Synuclein Toxicity and Aggregation Using a Humanized Yeast Model

Published on: November 25, 2022

Secondary assay to filter out undesirable compound mechanisms.

Main Results:

Successful identification of small molecules that rescue asyn toxicity in yeast.
Demonstration of scalable screening methods for large compound libraries (>100,000 compounds).

Conclusions:

Alpha-synuclein toxicity is modulated by Rab protein function.
Assay formats described are effective for identifying novel therapeutic compounds for Parkinson's disease.
Findings support Rab-mediated protein trafficking as a viable target for PD drug discovery.