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Related Experiment Videos

Soluble CD23 displays T-cell growth enhancing activity.

J C Lecron1, F Morel, J Tanzer

  • 1URA C.N.R.S. 1338, Hôpital La Milétrie, Poitiers, France.

Immunology
|November 1, 1991
PubMed
Summary

Soluble CD23 (sCD23) boosts T-cell colony formation from human blood cells. This suggests sCD23 plays a role in recruiting T-cell clones and potentially regulating IgE responses.

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Soluble CD23 (sCD23) is a cytokine involved in B-cell regulation.
  • Its role in T-cell activation and proliferation is not fully understood.

Purpose of the Study:

  • To investigate the effect of sCD23 on T-cell colony formation.
  • To determine the mechanisms underlying sCD23-mediated T-cell responses.

Main Methods:

  • T-cell colony assays using peripheral blood-derived T-colony cells.
  • Stimulation with phytohaemagglutinin (PHA) and interleukin-2 (IL-2).
  • Inhibition studies using anti-CD23 monoclonal antibody (mAb) and IgE.

Main Results:

  • sCD23 significantly enhanced secondary T-cell colony formation in a dose-dependent manner.

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  • The enhancing effect of sCD23 was independent of IL-1 and IL-4.
  • The effect was abolished by anti-CD23 mAb and IgE, indicating specificity.
  • sCD23 also increased the cloning frequency of primary T-cell colonies.

    • Conclusions:

    • sCD23 directly recruits T-cell clones from peripheral blood.
    • sCD23 may play an indirect role in the regulation of IgE responses.