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Visualization of IL-22-expressing Lymphocytes Using Reporter Mice
Published on: January 25, 2017
Interleukin 22 receptor 1 expression in pancreas islets
Makoto Shioya1, Akira Andoh, Satoe Kakinoki
1Department of Medicine, Shiga University of Medical Science, Otsu, Japan.
Pancreas
|April 1, 2008
Summary
Interleukin (IL)-22 receptor 1 (IL-22R1) is expressed in human pancreatic islets, particularly in insulin- and glucagon-producing cells. This suggests a role for T-cell signaling in regulating islet function.
Area of Science:
- Immunology
- Endocrinology
- Cell Biology
Background:
- Interleukin (IL)-22 is a T-cell-derived cytokine.
- IL-22 functions by binding to a receptor complex comprising IL-22 receptor 1 (IL-22R1) and IL-10 receptor 2 (IL-10R2).
Purpose of the Study:
- To investigate the expression of IL-22R1 in normal human pancreatic tissue.
- To determine the specific cell types within the pancreas that express IL-22R1.
Main Methods:
- Immunohistochemical analysis was performed on normal human pancreatic tissue samples (n=8).
- Standard immunohistochemical procedures were employed using anti-human IL-22R1 antibodies.
- Double-immunostaining was utilized to identify IL-22R1 expression in relation to specific pancreatic cell markers.
Main Results:
- IL-22R1 expression was detected within the islets of Langerhans in the normal human pancreas.
- IL-22R1 was notably absent in acinar cells and ductal epithelium.
- Double-staining confirmed that most insulin-expressing beta cells and glucagon-expressing alpha cells were positive for IL-22R1.
Conclusions:
- The islets of Langerhans represent the primary site of IL-22R1 expression in the human pancreas.
- T-cell-mediated immune responses may influence pancreatic islet cell function via IL-22 signaling.
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