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Updated: Jul 6, 2026

Measuring Oral Fatty Acid Thresholds, Fat Perception, Fatty Food Liking, and Papillae Density in Humans
Published on: June 4, 2014
Proposed modifications to AOAC 996.06, optimizing the determination of trans fatty acids: presentation of data
Brent Rozema1, Barbara Mitchell, Doug Winters
1Covance Laboratories Inc., 3301 Kinsman Blvd, Madison, WI 53704, USA. brent.rozema@covance.com
Abstract:
The increased focus on the accuracy of trans fatty acid data generated using current methodologies has resulted in research initiatives to optimize the quality of these assays. In this study, scientists combined the established methodology from AOAC 996.06 and the American Oil Chemists Society method Ce 1h-05, as well as other independent research. As a result, method modifications are proposed that could allow for a more accurate determination of trans fat than the current AOAC 996.06 method. Validation data from this study are presented. The authors encourage peer review and offer to facilitate a collaborative validation to update AOAC 996.06.
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