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Related Concept Videos

DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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Related Experiment Video

Updated: Jul 6, 2026

Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro
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Published on: July 5, 2017

Rapid isolation of red wine polymeric polyphenols by solid-phase extraction.

David W Jeffery1, Meagan D Mercurio, Markus J Herderich

  • 1The Australian Wine Research Institute, P.O. Box 197, Glen Osmond, SA 5064, Australia.

Journal of Agricultural and Food Chemistry
|April 2, 2008
PubMed
Summary

A new method rapidly isolates polymeric polyphenols from red wine using solid-phase extraction. This technique separates polyphenols into distinct fractions, aiding the study of these important wine compounds.

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Last Updated: Jul 6, 2026

Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro
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Published on: July 5, 2017

Microwave-Assisted Extraction of Phenolic Compounds and Antioxidants for Cosmetic Applications Using Polyol-Based Technology
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Microwave-Assisted Extraction of Phenolic Compounds and Antioxidants for Cosmetic Applications Using Polyol-Based Technology

Published on: August 23, 2024

Area of Science:

  • Food Chemistry
  • Analytical Chemistry

Background:

  • Red wine contains complex polyphenolic compounds, including monomeric, oligomeric, and polymeric forms.
  • Understanding the composition and properties of these polyphenols is crucial for wine quality and health impact studies.

Purpose of the Study:

  • To develop and validate a rapid technique for isolating polymeric polyphenols from red wine.
  • To separate red wine polyphenols into distinct fractions based on their chemical properties.

Main Methods:

  • Utilized a copolymer reversed-phase solid-phase extraction (SPE) cartridge.
  • Employed predominantly organic eluents for fractionation without sample pretreatment.
  • Analyzed phenolic fractions using High-Performance Liquid Chromatography (HPLC).

Main Results:

  • Successfully isolated three distinct phenolic fractions from red wine.
  • Separated monomeric/oligomeric polyphenols from polymeric polyphenols.
  • Characterized two unique polymeric polyphenol fractions differing in solubility and pigmentation, potentially related to wine age.

Conclusions:

  • The developed SPE method offers rapid isolation of discrete polymeric polyphenol fractions.
  • This technique simplifies the analysis of red wine polyphenols by removing interfering non-polymeric compounds.
  • The method facilitates further research into the role and characteristics of polymeric polyphenols in red wine.