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Comparative proteomic analysis of indioside D-triggered cell death in HeLa cells
Chi Chun Wong1, Ying Wang, Ka-Wing Cheng
1School of Biological Sciences, Department of Anatomy, The University of Hong Kong, Hong Kong SAR, China.
Journal of Proteome Research
|April 2, 2008
Summary
Indioside D from Solanum mammosum exhibits anticancer properties by inducing apoptosis in HeLa cells. This natural compound triggers mitochondrial dysfunction and activates both intrinsic and extrinsic cell death pathways.
Area of Science:
- Pharmacology
- Molecular Biology
- Cancer Research
Background:
- Medicinal plants are a valuable source of novel anticancer drug leads.
- Solanum mammosum is a plant known for its potential therapeutic properties.
Purpose of the Study:
- To investigate the antiproliferative activity and mechanism of action of Indioside D, a compound isolated from Solanum mammosum.
- To determine if Indioside D induces apoptosis in human cancer cell lines.
Main Methods:
- Proteomic analysis was performed on HeLa cells treated with Indioside D.
- Mitochondrial transmembrane potential (DeltaPsim) and reactive oxygen species (ROS) generation were measured.
- Caspase activation and the Fas death receptor pathway were assessed.
Main Results:
- Indioside D demonstrated antiproliferative activity against human cancer cell lines.
- Proteomic analysis indicated significant changes in energy production and oxidative stress pathways.
- Indioside D induced mitochondrial dysfunction, evidenced by DeltaPsim dissipation and ROS generation.
- Both caspase-dependent apoptosis and the Fas death receptor pathway, including caspase-8 and Bid cleavage, were activated.
Conclusions:
- Indioside D induces apoptosis in HeLa cells through a mechanism involving mitochondrial dysfunction.
- The compound activates both the intrinsic (mitochondrial) and extrinsic (Fas receptor) cell death pathways.
- Indioside D represents a promising lead compound for cancer drug development.
