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beta-Hexosaminidase expression in chick embryo fibroblasts in vitro
T Beccari1, M Bodo, E Becchetti
1Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Università di Perugia, Italy.
The International Journal of Biochemistry
|January 1, 1991
Summary
Two beta-hexosaminidase forms were identified in developing chick cells. Concanavalin-A influences hexosaminidase C levels and overall enzyme activity during development.
Area of Science:
- Biochemistry
- Developmental Biology
- Cell Biology
Background:
- Beta-hexosaminidase is a key enzyme involved in cellular processes.
- Understanding its regulation during development is crucial for biological insights.
Purpose of the Study:
- To investigate the forms of beta-hexosaminidase in developing chick embryo skin fibroblasts.
- To determine the effect of Concanavalin-A on enzyme activity and specific forms during development.
Main Methods:
- DEAE-cellulose chromatography was used to separate beta-hexosaminidase forms.
- Enzyme specific activity was measured during in vitro development.
- Concanavalin-A treatment was applied to assess its impact.
Main Results:
- Two beta-hexosaminidase forms, analogous to hexosaminidase A and C, were isolated.
- Beta-hexosaminidase specific activity increased during fibroblast development.
- Concanavalin-A treatment led to an increase in the neutral form (hexosaminidase C).
- Concanavalin-A also reduced the overall specific activity of beta-hexosaminidase.
Conclusions:
- Developing chick embryo skin fibroblasts express distinct beta-hexosaminidase forms.
- Concanavalin-A plays a regulatory role in the expression of hexosaminidase C and overall enzyme activity during development.