Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Problem-Solving

Problem-Solving

Effective problem-solving consists of two steps: 1. identifying the problem and 2. selecting the appropriate problem-solving strategy (i.e., a plan of action used to find a solution). Humans use four problem-solving strategies:

Hydraulic Jump: Problem Solving

Hydraulic Jump: Problem Solving

To analyze a hydraulic jump in a rectangular channel with a flow speed of 6 meters per second, follow these steps:Calculate Effective Upstream Velocity:When the downstream gate closes, a hydraulic jump forms, traveling upstream at 2 meters per second. This wave speed combines with the initial channel flow velocity, creating an effective upstream velocity.Identify Flow Velocities Before and After the Hydraulic Jump:Upstream of the hydraulic jump, the effective flow velocity includes both the...

Friction: Problem Solving

Friction: Problem Solving

Friction is an essential force that influences the motion of objects in daily life. Depending on the situation, it can be either beneficial or problematic. Consider a bus with a mass of three megagrams and its center of mass at a specific point, moving along a banked road at a constant speed. The coefficient of static friction between the tires and the road is 0.5. Find the maximum angle of the banked road at which the bus would not slip or tip.
Initially, a visual representation of the...

Screw: Problem Solving

Screw: Problem Solving

In mechanical engineering, the interaction between a threaded screw shaft and a plate gear involves analyzing the resisting torque on the plate gear that can be overpowered when a specific torsional moment is applied to the shaft. To better comprehend this concept, consider a generic situation with a threaded screw shaft with a given mean radius and lead and a plate gear with a specified mean radius. The coefficient of static friction between the screw and gear is also provided.
To evaluate the...

Problem Solving: Volume

Problem Solving: Volume

The volume of a fuel tank mounted on the wing of a jet aircraft can be modeled using the concept of solids of revolution. In this case, the tank is formed by rotating a two-dimensional region, defined by a mathematical function, about the x-axis. The region extends along the axis from zero to two meters, and the resulting three-dimensional shape is symmetric about the axis of rotation. Because the boundary curve lies directly against the axis, the disk method is an appropriate technique for...

Frames: Problem Solving I

Frames: Problem Solving I

Consider a jib crane with an external load suspended from the pulley. The dimensions of the crane members are shown in the figure. A systematic analysis of the frame structure is required to determine the reaction forces at the pin joints, assuming that the pulleys are frictionless.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Artificial Intelligence at the Intersection of Chemistry and Materials Science.

AI (Basel, Switzerland)·2026

Same author

The <i>BUD31</i> Homologous Gene in <i>Schizosaccharomyces pombe</i> Is Evolutionarily Conserved and Can Be Linked to Cellular Processes Regulated by the TOR Pathway.

Cells·2025

Same author

Exploring the Cka1 kinase interactome: unveiling novel potential regulatory roles of CKII kinase in S. pombe.

Biochemical and biophysical research communications·2025

Same author

Dbl2 interacts with helicases and an endonuclease to maintain the integrity of repetitive regions.

Scientific reports·2025

Same author

Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.

Biochemical and biophysical research communications·2024

Same author

Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Scientific reports·2024

Search research articles

Related Experiment Video

Updated: Jul 6, 2026

The Use of the Puzzle Box as a Means of Assessing the Efficacy of Environmental Enrichment

The Use of the Puzzle Box as a Means of Assessing the Efficacy of Environmental Enrichment

Published on: December 29, 2014

Solving the shugoshin puzzle.

Juraj Gregan¹, Mario Spirek, Cornelia Rumpf

¹Max F. Perutz Laboratories, University of Vienna, 1030 Vienna, Austria. juraj.gregan@univie.ac.at

Trends in Genetics : TIG

|April 2, 2008

Summary

Shugoshin-protein phosphatase 2A complexes protect centromeric cohesin during yeast meiosis I. This conserved mechanism explains why protection occurs specifically in meiosis I, not meiosis II or mitosis.

Area of Science:

Cell Biology
Genetics
Molecular Biology

Background:

Shugoshin proteins and protein phosphatase 2A (PP2A) form a complex critical for chromosome segregation.
This complex safeguards centromeric cohesin from premature cleavage by separase during meiosis I.
The precise regulation of cohesin cleavage is essential for accurate chromosome distribution in eukaryotes.

Purpose of the Study:

To elucidate the mechanism by which the shugoshin-PP2A complex specifically protects centromeric cohesin during meiosis I.
To address the long-standing question of why this protective function is restricted to meiosis I and not observed in meiosis II or mitosis.
To provide a conserved molecular model for meiotic chromosome regulation.

Main Methods:

Investigated the interaction between shugoshin proteins and PP2A in yeast models.

More Related Videos

The Innovation Arena: A Method for Comparing Innovative Problem-Solving Across Groups

The Innovation Arena: A Method for Comparing Innovative Problem-Solving Across Groups

Published on: May 13, 2022

New Variations for Strategy Set-shifting in the Rat

New Variations for Strategy Set-shifting in the Rat

Published on: January 23, 2017

Related Experiment Videos

Last Updated: Jul 6, 2026

The Use of the Puzzle Box as a Means of Assessing the Efficacy of Environmental Enrichment

The Use of the Puzzle Box as a Means of Assessing the Efficacy of Environmental Enrichment

Published on: December 29, 2014

The Innovation Arena: A Method for Comparing Innovative Problem-Solving Across Groups

The Innovation Arena: A Method for Comparing Innovative Problem-Solving Across Groups

Published on: May 13, 2022

New Variations for Strategy Set-shifting in the Rat

New Variations for Strategy Set-shifting in the Rat

Published on: January 23, 2017

Analyzed the role of the shugoshin-PP2A complex in preventing separase-mediated cohesin cleavage.

Compared the function of the complex across different cell division stages (meiosis I, meiosis II, mitosis).

Main Results:

Confirmed that the shugoshin-PP2A complex is essential for protecting centromeric cohesin during meiosis I in yeast.
Demonstrated evolutionary conservation of this protective mechanism from yeast to mammals.
Developed a model explaining the meiosis I-specific function of the shugoshin-PP2A complex in cohesin protection.

Conclusions:

The shugoshin-PP2A complex plays a conserved role in ensuring accurate chromosome segregation by protecting centromeric cohesin during meiosis I.
The study provides a mechanistic explanation for the temporal specificity of cohesin protection, resolving a key question in cell division.
This finding deepens our understanding of the molecular basis of meiosis and its regulation in eukaryotes.