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Updated: Jul 6, 2026

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters
Published on: March 9, 2016
Comparison of transcription-mediated amplification and growth-based methods for the quantitation of Enterococcus
Ceri A Morris1, A Denene Blackwood, Marek Kirs
1MLT Research Ltd., 5 Chiltern Close, Cardiff CF14 5DL, United Kingdom. ceri.morris@mltresearch.com
Abstract:
An assay based on transcription-mediated amplification (TMA) technology was used to quantitate Enterococcus fecal indicator bacteria in environmental water samples. The results generated by this and two growth-based methods relative to the 104 most-probable-number or CFU-per-100-ml threshold show that the three methods are in good qualitative agreement when tested against a range of water samples taken from different locations. The results demonstrate sensitive and rapid detection (approximately 4 h from sample collection to result) and quantitation of Enterococcus bacteria compared to the results with the growth-based methods.
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